Purification and Properties of Inducible Hydroxyproline 2-epimerase from Pseudomonas.

In the pathway of hydrosyproline metabolism by an inducible strain of Pseudomonas (1)) an initial reaction involves the reversible epimerization of hydroxy-L-proline to allohydroxy-n-proline by racemization at carbon 2. In subsequent steps of this sequence, allohydrosy-n-proline is oxidized to Al-pyrrolinelhydroxy2carboxylate (2) and the latter compound is oxidized to ar-ketoglutarate through an intermediate which has been identified as ol-ketoglutaric semialdehyde (9). Preliminary studies (1) of the epimerase showed that the enzyme was inducible, was not sedimented from sonic extracts at 100,000 X g, and appeared to cat.alyze rather specifically the two reactions,